XDH Polyklonaler Antikörper

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• KD/KO Validated

XDH Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 55156-1-AP

Synonyme

xanthine dehydrogenase, Xanthine Oxidase, Xanthine oxidoreductase, XD, XDH, XDHA, XO, XOR

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 55156-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below

Produktinformation

55156-1-AP bindet in WB, IHC, ELISA XDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: xanthine dehydrogenase
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 147-150 kDa
• GenBank-Zugangsnummer: NM_000379
• Gene symbol: XDH
• Gene ID (NCBI): 7498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XDH, also named as XDHA, XO and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro.This gene encodes a protein of 150 kDa and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage(PMID:9989587). In addition, the full length polypeptide can be also partially processed into a 87 kDa and 59 kDa fragments(PMID:21528298). It also can exsit as a homodimer(PMID:9989587). This antibody is specific to XDH.

Publikationen

Species	Application	Title
mouse,human	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu
mouse	WB	Sci Rep; Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.; Authors - Huihui Li
human	WB	Sci Rep; Peptide SS-31 upregulates frataxin expression and improves the quality of mitochondria: implications in the treatment of Friedreich ataxia.; Authors - Hongting Zhao
rat	WB	J Mol Cell Cardiol; Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.; Authors - Yusuke Igaki