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XBP1S/XBP1U Polyklonaler Antikörper

XBP1S/XBP1U Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24168-1-AP

Synonyme

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1s, XBP1S/XBP1U, XBP1U, XBP2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, Raji-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Immunhistochemie (IHC)IHC : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

24168-1-AP bindet in WB, IHC, FC, ELISA XBP1S/XBP1U und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen XBP1S/XBP1U fusion protein Ag21454
Vollständiger Name X-box binding protein 1
Berechnetes Molekulargewicht 261 aa, 29 kDa
Beobachtetes Molekulargewicht ~32 kDa; ~60 kDa
GenBank-ZugangsnummerBC000938
Gene symbol XBP1
Gene ID (NCBI) 7494
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.

Protokolle

Produktspezifische Protokolle
WB protocol for XBP1S/XBP1U antibody 24168-1-APProtokoll herunterladen
IHC protocol for XBP1S/XBP1U antibody 24168-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Bioorg Chem

Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress.

Authors - Tongxi Li
humanWB

J Virol

Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.

Authors - Yanxia Huang
  • KD Validated
mouseWB

J Zhejiang Univ Sci B

Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure

Authors - Xue YANG
humanWB

Cell Death Discov

24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death.

Authors - Yasuomi Urano
humanWB

J Exp Clin Cancer Res

Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress.

Authors - Weiwei Sheng
mouseWB

ACS Appl Mater Interfaces

Probiotic-Functionalized Silk Fibroin/Sodium Alginate Scaffolds with Endoplasmic Reticulum Stress-Relieving Properties for Promoted Scarless Wound Healing

Authors - Zhaona Dou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,