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XBP1S/XBP1U Polyklonaler Antikörper
XBP1S/XBP1U Polyklonal Antikörper für FC, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 24168-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, Raji-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in FC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 8 publications below |
IHC | See 1 publications below |
Produktinformation
24168-1-AP bindet in WB, IHC, FC, ELISA XBP1S/XBP1U und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | XBP1S/XBP1U fusion protein Ag21454 |
Vollständiger Name | X-box binding protein 1 |
Berechnetes Molekulargewicht | 261 aa, 29 kDa |
Beobachtetes Molekulargewicht | ~32 kDa; ~60 kDa |
GenBank-Zugangsnummer | BC000938 |
Gene symbol | XBP1 |
Gene ID (NCBI) | 7494 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-affinitätsgereinigt |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for XBP1S/XBP1U antibody 24168-1-AP | Protokoll herunterladen |
IHC protocol for XBP1S/XBP1U antibody 24168-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Bioorg Chem Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress. | ||
J Virol Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.
| ||
J Zhejiang Univ Sci B Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure | ||
Cell Death Discov 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death. | ||
J Exp Clin Cancer Res Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress. | ||
ACS Appl Mater Interfaces Probiotic-Functionalized Silk Fibroin/Sodium Alginate Scaffolds with Endoplasmic Reticulum Stress-Relieving Properties for Promoted Scarless Wound Healing |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
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