WRAP53 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WRAP53 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 14761-1-AP

Synonyme

TCAB1, WDR79, WRAP53

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Galerie der Validierungsdaten

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  WB analysis using 14761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14761-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14761-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14761-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14761-1-AP

  IP Result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, humanes Nierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

14761-1-AP bindet in WB, IP, IHC, IF, ELISA WRAP53 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WRAP53 fusion protein Ag6522
• Vollständiger Name: WD repeat containing, antisense to TP53
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 72-75 kDa
• GenBank-Zugangsnummer: BC002336
• Gene symbol: WRAP53
• Gene ID (NCBI): 55135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Genes Dev; The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.; Authors - Sofia Henriksson
human	WB	Nat Chem Biol; Observation of processive telomerase catalysis using high-resolution optical tweezers.; Authors - Eric M Patrick
human	WB	Oncogenesis; RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.; Authors - Qunsong Tan ; KD Validated
	WB	Aging (Albany NY); Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.; Authors - Xing-Hua Pan
human	WB,IF	RNA Biol; Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.; Authors - Christos Coucoravas ; KD Validated
human	WB	Cancer Cell Int; Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.; Authors - Jing Niu