WNT4 Polyklonaler Antikörper

WNT4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14371-1-AP

Synonyme

Protein Wnt 4, SERKAL, WNT 4, WNT4

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Galerie der Validierungsdaten

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  WB analysis using 14371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14371-1-AP (WNT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14371-1-AP bindet in WB, IHC, IF, ELISA WNT4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT4 fusion protein Ag5741
• Vollständiger Name: wingless-type MMTV integration site family, member 4
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC057781
• Gene symbol: WNT4
• Gene ID (NCBI): 54361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

Publikationen

Species	Application	Title
human	WB	Endocrinology; PMID: 31748790; Authors - Alison M Neff
rat		Int J Clin Exp Pathol; Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.; Authors - Junyi Hua
mouse	IHC	Neurochem Res; Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.; Authors - Yu Li L
human	IF	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
mouse	IHC	BMC Complement Med Ther; Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.; Authors - Bing Cao
human	WB,IHC	Eur J Pharmacol; HucMSC exosomes attenuate partial bladder outlet obstruction-induced renal injury and cell proliferation via the Wnt/β-catenin pathway; Authors - Zhaoying Wang