WNK1 Monoklonaler Antikörper

WNK1 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6A10

Kat-Nr. : 68149-1-Ig

Synonyme

Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1

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Galerie der Validierungsdaten

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  WB analysis using 68149-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using 68149-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68149-1-Ig bindet in WB, FC, ELISA WNK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WNK1 fusion protein Ag28044
• Vollständiger Name: WNK lysine deficient protein kinase 1
• Berechnetes Molekulargewicht: 251 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC021121
• Gene symbol: WNK1
• Gene ID (NCBI): 65125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.