WISP3 Polyklonaler Antikörper

WISP3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26406-1-AP

Synonyme

CCN family member 6, CCN6, LIBC, PPAC, PPD, WISP 3, WISP3

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Galerie der Validierungsdaten

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  WB analysis using 26406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26406-1-AP (WISP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26406-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26406-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26406-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26406-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26406-1-AP bindet in WB, IHC, ELISA WISP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WISP3 fusion protein Ag23999
• Vollständiger Name: WNT1 inducible signaling pathway protein 3
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC105941
• Gene symbol: WISP3
• Gene ID (NCBI): 8838
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WISP3, also known as CCN6. Localized to the Mitochondria, and It is reported that CCN6 is located in the nucleus of breast cancer (PMID: 21262769). It is mainly expressed in adult kidney, testis and fetal kidney, and weakly expressed in placenta, ovary, prostate and small intestine (PMID: 9843955, PMID: 10471507). This gene is overexpressed in colon tumors, and it may be located downstream of WNT1 signaling pathway related to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Also it plays an important role in mitochondrial electron transfer and mitochondrial respiration, and may be important in bone growth and cartilage homeostasis after normal birth through its regulation of mitochondrial function (PMID: 27252383, PMID: 10471507). The calculated molecular weight of WISP3 is 39 kDa, and this antibody can recognize the 41 kDa isoform of WISP3.