WFS1 Polyklonaler Antikörper

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• KD/KO Validated

WFS1 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 594 Fluorescent Dye

Kat-Nr. : CL594-26995

Synonyme

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

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Galerie der Validierungsdaten

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  IF Staining of rat brain using CL594-26995

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 594 WFS1 antibody (CL594-26995) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-26995

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 594 WFS1 antibody (CL594-26995) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-26995

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 594 WFS1 antibody (CL594-26995) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-26995 bindet in IF WFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WFS1 fusion protein Ag25724
• Vollständiger Name: Wolfram syndrome 1 (wolframin)
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• GenBank-Zugangsnummer: BC030130
• Gene symbol: WFS1
• Gene ID (NCBI): 7466
• Konjugation: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 594 nm / 615 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.