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WFS1 Polyklonaler Antikörper
WFS1 Polyklonal Antikörper für IF, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 26995-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IHC | Rattenhirngewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | Maushirngewebe, HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 3 publications below |
IF | See 8 publications below |
Produktinformation
26995-1-AP bindet in WB, IHC, IF, ELISA WFS1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | WFS1 fusion protein Ag25724 |
Vollständiger Name | Wolfram syndrome 1 (wolframin) |
Berechnetes Molekulargewicht | 890 aa, 100 kDa |
GenBank-Zugangsnummer | BC030130 |
Gene symbol | WFS1 |
Gene ID (NCBI) | 7466 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.
Protokolle
Produktspezifische Protokolle | |
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IHC protocol for WFS1 antibody 26995-1-AP | Protokoll herunterladen |
IF protocol for WFS1 antibody 26995-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Sci Transl Med Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice. | ||
Nat Commun WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.
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Prog Neurobiol The fasciola cinereum subregion of the hippocampus is important for the acquisition of visual contextual memory. | ||
Elife The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes | ||
Acta Neuropathol Commun Wolfram syndrome 1b mutation suppresses Mauthner-cell axon regeneration via ER stress signal pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jordan (Verified Customer) (01-25-2021) | Used free floating staining 16 hours in primary and 6 hours in secondary (Alexa 488+) at 4 degrees C. Antibody does a perfect job of labelling MEC layer 2 pyramidal neurons, alongside Parasubiculum neurons, some neurons in the subiculum and the majority of CA1 pyramidal neurons as widely reported in the literature
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