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WFS1 Polyklonaler Antikörper

WFS1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26995-1-AP

Synonyme

WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN



Geprüfte Anwendungen

Erfolgreiche Detektion in IHCRattenhirngewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaushirngewebe, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

26995-1-AP bindet in WB, IHC, IF, ELISA WFS1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen WFS1 fusion protein Ag25724
Vollständiger Name Wolfram syndrome 1 (wolframin)
Berechnetes Molekulargewicht 890 aa, 100 kDa
GenBank-ZugangsnummerBC030130
Gene symbol WFS1
Gene ID (NCBI) 7466
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Protokolle

Produktspezifische Protokolle
IHC protocol for WFS1 antibody 26995-1-APProtokoll herunterladen
IF protocol for WFS1 antibody 26995-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Sci Transl Med

Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice.

Authors - Jean-Christophe Delpech
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
human,ratWB,IF

Nat Commun

WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.

Authors - Linlin Wang
  • KO Validated
ratIHC

Prog Neurobiol

The fasciola cinereum subregion of the hippocampus is important for the acquisition of visual contextual memory.

Authors - Seong-Beom Park
mouseIHC

Elife

The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

Authors - Xiong Yang
zebrafishWB

Acta Neuropathol Commun

Wolfram syndrome 1b mutation suppresses Mauthner-cell axon regeneration via ER stress signal pathway

Authors - Zongyi Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jordan (Verified Customer) (01-25-2021)

Used free floating staining 16 hours in primary and 6 hours in secondary (Alexa 488+) at 4 degrees C. Antibody does a perfect job of labelling MEC layer 2 pyramidal neurons, alongside Parasubiculum neurons, some neurons in the subiculum and the majority of CA1 pyramidal neurons as widely reported in the literature

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Brain/Pyramidal Neurons