WEE2 Polyklonaler Antikörper

WEE2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55119-1-AP

Synonyme

WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, Wee1 like protein kinase 2, WEE1B, Wee1B kinase, WEE2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, humanes Eierstockgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55119-1-AP bindet in WB,ELISA WEE2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name WEE1 homolog 2 (S. pombe)
Berechnetes Molekulargewicht 63 kDa
Beobachtetes Molekulargewicht 63-66 kDa
GenBank-ZugangsnummerNM_001105558
Gene symbol WEE2
Gene ID (NCBI) 494551
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.

Protokolle

Produktspezifische Protokolle
WB protocol for WEE2 antibody 55119-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen