WEE2 Polyklonaler Antikörper
WEE2 Polyklonal Antikörper für WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB,ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55119-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Jurkat-Zellen, humanes Eierstockgewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
55119-1-AP bindet in WB,ELISA WEE2 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | WEE1 homolog 2 (S. pombe) |
Berechnetes Molekulargewicht | 63 kDa |
Beobachtetes Molekulargewicht | 63-66 kDa |
GenBank-Zugangsnummer | NM_001105558 |
Gene symbol | WEE2 |
Gene ID (NCBI) | 494551 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3. |
Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for WEE2 antibody 55119-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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