WDR61 Polyklonaler Antikörper

WDR61 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22536-1-AP

Synonyme

REC14, SKI8, WD repeat domain 61, WDR61

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Galerie der Validierungsdaten

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  WB analysis using 22536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22536-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22536-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22536-1-AP

  IP Result of anti-WDR61 (IP:22536-1-AP, 4ug; Detection:22536-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22536-1-AP

  Immunofluorescent analysis of HeLa cells using 22536-1-AP (WDR61 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

22536-1-AP bindet in WB, IP, IHC, IF, ELISA WDR61 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR61 fusion protein Ag18443
• Vollständiger Name: WD repeat domain 61
• Berechnetes Molekulargewicht: 305 aa, 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC010080
• Gene symbol: WDR61
• Gene ID (NCBI): 80349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.

Publikationen

Species	Application	Title
human	WB	Sci Adv; FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer; Authors - Yajie Liu
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
		Mol Cell; SPT6 functions in transcriptional pause/release via PAF1C recruitment; Authors - Yuki Aoi
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao