TrpRS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TrpRS Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16081-1-AP

Synonyme

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, Tryptophanyl-tRNA synthetase, WARS, WARS1, WRS

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Galerie der Validierungsdaten

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  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16081-1-AP

  IP Result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug.

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  IHC staining of human ovary tumor using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16081-1-AP

  Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16081-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using TrpRS antibody (16081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 16081-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug TrpRS antibody (16081-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, JAR-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

16081-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TrpRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TrpRS fusion protein Ag9079
• Vollständiger Name: tryptophanyl-tRNA synthetase
• Berechnetes Molekulargewicht: 471 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC017489
• Gene symbol: WARS
• Gene ID (NCBI): 7453
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	J Immunother Cancer; Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.; Authors - Rui Qin ; KO Validated