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VTN Monoklonaler Antikörper

VTN Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2C8

Kat-Nr. : 66398-1-Ig

Synonyme

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human plasma using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of human plasma using 66398-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig plasma using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of pig plasma using 66398-1-Ig

    pig plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit serum using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of rabbit serum using 66398-1-Ig

    rabbit serum were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:350 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using VTN Monoclonal antibody (66398-1-Ig)

    IF Staining of NIH/3T3 using 66398-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VTN antibody (66398-1-Ig, Clone: 1D2C8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plasmagewebe, Hausschwein-Plasmagewebe, Kaninchen-Serumgewebe
Erfolgreiche Detektion in IHChumanes Hodengewebe, humanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:10000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

66398-1-Ig bindet in WB, IHC, IF, ELISA VTN und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus
In Publikationen genannte ReaktivitätHausschwein
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen VTN fusion protein Ag8443
Vollständiger Name vitronectin
Berechnetes Molekulargewicht 478 aa, 54 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC005046
Gene symbol VTN
Gene ID (NCBI) 7448
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Protokolle

Produktspezifische Protokolle
WB protocol for VTN antibody 66398-1-IgProtokoll herunterladen
IHC protocol for VTN antibody 66398-1-IgProtokoll herunterladen
IF protocol for VTN antibody 66398-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
pigIHC

Int J Mol Sci

Large-Scale Protein Analysis of Experimental Retinal Artery Occlusion

Authors - Nanna Vestergaard
View Article