VTI1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VTI1B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 14495-1-AP

Synonyme

VTI1, VTI1 LIKE, Vti1 rp1, VTI1B, VTI1L, VTI1L1, VTI2

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Galerie der Validierungsdaten

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  WB analysis using 14495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14495-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 14495-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14495-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14495-1-AP

  IP Result of anti-VTI1B (IP:14495-1-AP, 3ug; Detection:14495-1-AP 1:1000) with HeLa cells lysate 2500ug.

• 

  IHC staining of human liver cancer using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 14495-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, humanes Lebergewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

14495-1-AP bindet in WB, IP, IHC, IF, ELISA VTI1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VTI1B fusion protein Ag5906
• Vollständiger Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC003142
• Gene symbol: VTI1B
• Gene ID (NCBI): 10490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).

Publikationen

Species	Application	Title
human	WB	Autophagy; The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.; Authors - Takashi Nozawa ; KD Validated
human	WB	mBio; Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection; Authors - Sandhya Ganesan
mouse	IF	Front Cell Dev Biol; The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation; Authors - Amna Music

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had a specific band at VTI1B's predicted MW ~28 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart