VPS8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS8 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15079-1-AP

Synonyme

KIAA0804, VPS8

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15079-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15079-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15079-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15079-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15079-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15079-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse brain using 15079-1-AP

  IP Result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 15079-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

15079-1-AP bindet in WB, IP, IF, ELISA VPS8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS8 fusion protein Ag7151
• Vollständiger Name: vacuolar protein sorting 8 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 151 kDa
• GenBank-Zugangsnummer: BC001001
• Gene symbol: VPS8
• Gene ID (NCBI): 23355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

Publikationen

Species	Application	Title
human	WB	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
human	WB, IP	Traffic; Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations.; Authors - Enrico Daniele Perini
human	WB	Biochem J; VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.; Authors - Morag R Hunter
human	WB	Sci Rep; HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis; Authors - Seigo Terawaki ; KO Validated