VPS4B Monoklonaler Antikörper

VPS4B Monoklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H3G7

Kat-Nr. : 68443-1-Ig

Synonyme

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

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Galerie der Validierungsdaten

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  WB analysis using 68443-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 68443-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 68443-1-Ig

  IP result of anti-VPS4B(IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug.

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  IF Staining of HeLa using 68443-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68443-1-Ig bindet in WB, IP, IF, ELISA VPS4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VPS4B fusion protein Ag11610
• Vollständiger Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 444 aa, 49 kDa
• Beobachtetes Molekulargewicht: ~50 kDa
• GenBank-Zugangsnummer: BC039574
• Gene symbol: VPS4B
• Gene ID (NCBI): 9525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)