VPS16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS16 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 17776-1-AP

Synonyme

hVPS16, VPS16

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Galerie der Validierungsdaten

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  WB analysis using 17776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17776-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17776-1-AP

  IP Result of anti-VPS16 (IP:17776-1-AP, 3ug; Detection:17776-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IF: See 9 publications below

Produktinformation

17776-1-AP bindet in WB, IP, IHC, IF, ELISA VPS16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS16 fusion protein Ag12202
• Vollständiger Name: vacuolar protein sorting 16 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 839 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC073959
• Gene symbol: VPS16
• Gene ID (NCBI): 64601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
mouse	WB	Mol Cell; Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.; Authors - Ian G Ganley ; KD Validated
mouse	WB	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
mouse	IF	Redox Biol; Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.; Authors - Guangbi Li