VPS13C Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS13C Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 28676-1-AP

Synonyme

FLJ21361, KIAA1421, VPS13C

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Galerie der Validierungsdaten

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  WB analysis using 28676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28676-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28676-1-AP bindet in WB, IHC, IF, ELISA VPS13C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS13C fusion protein Ag29434
• Vollständiger Name: vacuolar protein sorting 13 homolog C (S. cerevisiae)
• Beobachtetes Molekulargewicht: 430 kDa
• GenBank-Zugangsnummer: NM_001018088
• Gene symbol: VPS13C
• Gene ID (NCBI): 54832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling.; Authors - William Hancock-Cerutti
human,mouse	WB,IHC,IF	iScience; Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1; Authors - Xiangyu Tan ; KD Validated
human	WB	Contact (Thousand Oaks); VPS13A and VPS13C Influence Lipid Droplet Abundance; Authors - Shuliang Chen ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (01-25-2021)

HEK293T lysates (10ug) ran on 4-12% gradient gel prior to transfer to nitrocellulose membrane. Membrane incubated overnight at 4 degrees with antibody (1:250). Goat anti-rabbit HRP secondary antibody (1:10,000) incubated with membrane for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

VPS13C Antibody Western Blot validation (1:250 dilution) in HEK293T (Cat no:28676-1-AP)