VPS11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS11 Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19140-1-AP

Synonyme

END1, hVPS11, PEP5, RING finger protein 108, RNF108, VPS11

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Galerie der Validierungsdaten

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  WB analysis using 19140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19140-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19140-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19140-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 19140-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19140-1-AP

  IP Result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  FC experiment of HepG2 using 19140-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VPS11 antibody (19140-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Nierengewebe, K-562-Zellen, Maushirngewebe, Maus-Pankreasgewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

19140-1-AP bindet in WB, IP, IF, FC, ELISA VPS11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS11 fusion protein Ag6227
• Vollständiger Name: vacuolar protein sorting 11 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 108-112 kDa
• GenBank-Zugangsnummer: BC065563
• Gene symbol: VPS11
• Gene ID (NCBI): 55823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publikationen

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R ; KO Validated
human	WB	J Mol Biol; Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.; Authors - Morag R Hunter
human	WB, IF	J Biol Chem; Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.; Authors - Rik van der Kant
human	IF,WB	J Cell Sci; The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.; Authors - Magdalena Banach-Orłowska ; KD Validated
human	WB,IP	Biochem J; VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.; Authors - Morag R Hunter