VMA21 Polyklonaler Antikörper

VMA21 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21921-1-AP

Synonyme

MEAX, VMA21, XMEA

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  WB analysis using 21921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 21921-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21921-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21921-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 21921-1-AP

  IP result of anti-VMA21 (IP:21921-1-AP, 3ug; Detection:21921-1-AP 1:1000) with MCF-7 cells lysate 4000ug.

• 

  IHC staining of mouse brain using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21921-1-AP (VMA21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

21921-1-AP bindet in WB, IHC, IP, ELISA VMA21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VMA21 fusion protein Ag16439
• Vollständiger Name: VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 101 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC105694
• Gene symbol: VMA21
• Gene ID (NCBI): 203547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
human,mouse	WB	J Transl Med; Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis.; Authors - Hong-Yu Shen
mouse	WB	Autophagy; Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice; Authors - Jia-Jun Wu