VHL Polyklonaler Antikörper

VHL Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16538-1-AP

Synonyme

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1, Von Hippe-Lindau



Geprüfte Anwendungen

Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 4 publications below
IFSee 1 publications below

Produktinformation

16538-1-AP bindet in IHC, IF, ELISA VHL und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VHL fusion protein Ag9843
Vollständiger Name von Hippel-Lindau tumor suppressor
Berechnetes Molekulargewicht 172 aa, 20 kDa
GenBank-ZugangsnummerBC058831
Gene symbol VHL
Gene ID (NCBI) 7428
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Protokolle

Produktspezifische Protokolle
IHC protocol for VHL antibody 16538-1-APProtokoll herunterladen
IF protocol for VHL antibody 16538-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseIHC

Respir Res

Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.

Authors - Changhui Yu
human

Oncotarget

The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.

Authors - Fabao Liu
humanIHC

Front Oncol

Risk Scores Based on Six Survival-Related RNAs in a Competing Endogenous Network Composed of Differentially Expressed RNAs Between Clear Cell Renal Cell Carcinoma Patients Carrying Wild-Type or Mutant Von Hippel-Lindau Serve Well to Predict Malignancy and Prognosis.

Authors - Rui Zhu
human,mouseIHC

Front Mol Biosci

Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.

Authors - Yanting Shi
human,mouseIHC,IF

Cancer Med

UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma

Authors - Junyi Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jingwen (Verified Customer) (01-27-2020)

This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Cancer tissue
FH

Jie (Verified Customer) (01-27-2020)

achieved positive staining in liver cancer cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:200
FH

Lunfeng (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,