VEGFD Polyklonaler Antikörper

VEGFD Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 26915-1-AP

Synonyme

c Fos induced growth factor, FIGF, VEGF D, VEGFD

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Galerie der Validierungsdaten

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  WB analysis using 26915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26915-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, MCF-7-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Herzgewebe, humanes Kolonkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

26915-1-AP bindet in WB, IHC, IF, ELISA VEGFD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFD fusion protein Ag25552
• Vollständiger Name: c-fos induced growth factor (vascular endothelial growth factor D)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC027948
• Gene symbol: FIGF
• Gene ID (NCBI): 2277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

Publikationen

Species	Application	Title
human	WB,IHC	Gastric Cancer; Cathepsin L promotes angiogenesis by regulating the CDP/Cux/VEGF-D pathway in human gastric cancer.; Authors - Tao Pan
mouse	IHC	Cereb Cortex; Prolonged Consumption of Sweetened Beverages Lastingly Deteriorates Cognitive Functions and Reward Processing in Mice.; Authors - Héloïse Hamelin
rat	IF	FASEB J; VEGF-D-mediated signaling in tendon cells is involved in degenerative processes.; Authors - Herbert Tempfer
mouse	IHC	Ann Transl Med; Clostridium butyricum alleviates dextran sulfate sodium-induced experimental colitis and promotes intestinal lymphatic vessel regeneration in mice.; Authors - Xing Chen
mouse	IHC	BMC Complement Med Ther; Effects of plant-based medicinal food on postoperative recurrence and lung metastasis of gastric cancer regulated by Wnt/β-catenin-EMT signaling pathway and VEGF-C/D-VEGFR-3 cascade in a mouse model; Authors - Lin Tian
mouse,human	WB,IF	Biochim Biophys Acta Mol Basis Dis; WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization; Authors - Yanhui Bai