VDR Monoklonaler Antikörper

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• KD/KO Validated

VDR Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

CloneNo.

1A9C1

Kat-Nr. : 67192-1-Ig

Synonyme

NR1I1, VDR, Vitamin D3 receptor

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Galerie der Validierungsdaten

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  WB analysis using 67192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, 4T1-Zellen, COLO 320-Zellen, HeLa-Zellen, NCCIT-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

67192-1-Ig bindet in WB, IF, ChIP, ELISA VDR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VDR fusion protein Ag28188
• Vollständiger Name: vitamin D (1,25- dihydroxyvitamin D3) receptor
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC060832
• Gene symbol: VDR
• Gene ID (NCBI): 7421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vitamin D receptor (VDR), also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25(OH)2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A). A disorder of vitamin D metabolism results in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. The VDR exists two isoform with the MV 48 kDa and 54 kDa.

Publikationen

Species	Application	Title
mouse	WB	Antioxidants (Basel); Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis; Authors - Juan Wei
human	WB	Front Pharmacol; Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway; Authors - Xiaowei Sun
	IF	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.; Authors - Shanhe Wang
human	WB,IF	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng
mouse	WB	Phytomedicine; Asperuloside inhibited epithelial-mesenchymal transition in colitis associated cancer via activation of vitamin D receptor.; Authors - Yingyu Lu ; KD Validated
human	WB	Front Microbiol; Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis via Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which Is Regulated by METTL3 and YTHDF3.; Authors - Wenbo Zhu ; KD Validated