VAV3 Polyklonaler Antikörper

VAV3 Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30291-1-AP

Synonyme

FLJ40431, VAV 3, VAV3

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Galerie der Validierungsdaten

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  WB analysis using 30291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30291-1-AP (VAV3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 30291-1-AP

  IP result of anti-VAV3(IP:30291-1-AP, 4ug; Detection:30291-1-AP 1:8000) with Jurkat cells lysate 1560 ug.

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  FC experiment of Jurkat using 30291-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human VAV3 (30291-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, THP-1-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30291-1-AP bindet in WB, IP, FC, ELISA VAV3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAV3 fusion protein Ag33022
• Vollständiger Name: vav 3 guanine nucleotide exchange factor
• Berechnetes Molekulargewicht: 98kd
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: NM_006113
• Gene symbol: VAV3
• Gene ID (NCBI): 10451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vav3, a member of Dbl family which is also the activator of GTP kinase of Rho family, can activate the expression of RhoA, Racl and Cdc42 (PMID:28285969). Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis (PMID:28285969). Vav3 is a multiple function protein with both signaling molecule and coactivator activities. Vav3 oncogene is overexpressed in androgen-independent prostate cancer cells relative to their androgen-dependent counterparts and contribute to development of hormone refractory prostate cancer (PMID:21455584).