VAV2 Polyklonaler Antikörper

VAV2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21924-1-AP

Synonyme

VAV 2, VAV2

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Galerie der Validierungsdaten

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  WB analysis using 21924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21924-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21924-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21924-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21924-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21924-1-AP

  IP result of anti-VAV2(IP:21924-1-AP, 4ug; Detection:21924-1-AP 1:2000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human pancreas using 21924-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21924-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 21924-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21924-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21924-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21924-1-AP (VAV2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, Maushirngewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21924-1-AP bindet in WB, IP, IHC, IF, ELISA VAV2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAV2 fusion protein Ag16458
• Vollständiger Name: vav 2 guanine nucleotide exchange factor
• Berechnetes Molekulargewicht: 839 aa, 97 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC132967
• Gene symbol: VAV2
• Gene ID (NCBI): 7410
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	FASEB J; Mesenchymal stem cells promote endothelial progenitor cell migration, vascularization, and bone repair in tissue-engineered constructs via activating CXCR2-Src-PKL/Vav2-Rac1.; Authors - Zhilin Li