VAMP7/TI-VAMP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VAMP7/TI-VAMP Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, Blocking, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22268-1-AP

Synonyme

SYBL1, Synaptobrevin like protein 1, Tetanus insensitive VAMP, TI VAMP, TI-VAMP, VAMP 7, VAMP7, VAMP7/TI-VAMP

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Galerie der Validierungsdaten

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  WB analysis using 22268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22268-1-AP

  IP Result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 22268-1-AP

  Immunofluorescent analysis of HepG2 cells using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 22268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

22268-1-AP bindet in WB, IP, IHC, IF, Blocking, ELISA VAMP7/TI-VAMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: vesicle-associated membrane protein 7
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: NM_005638
• Gene symbol: VAMP7
• Gene ID (NCBI): 6845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP7, also named as SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is required for calcium regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.

Publikationen

Species	Application	Title
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan ; KO Validated
human	WB	Cancer Res Commun; Combination of a New Oral Demethylating Agent, OR2100, and Venetoclax for Treatment of Acute Myeloid Leukemia; Authors - Kazuharu Kamachi ; KD Validated
mouse	WB,IF	Biochem Biophys Res Commun; VAMP7 knockdown in secretory granules impairs CCL2 secretion in mast cells; Authors - Marin Sakamoto ; KD Validated
rat	WB	Small; Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.; Authors - Jia Liu