VAC14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VAC14 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15771-1-AP

Synonyme

ArPIKfyve, Protein VAC14 homolog, Tax1 binding protein 2, TAX1BP2, TRX, VAC14, Vac14 homolog (S. cerevisiae)

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Galerie der Validierungsdaten

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  WB analysis of human spleen using 15771-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15771-1-AP (VAC14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15771-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15771-1-AP (VAC14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15771-1-AP

  IP Result of anti-VAC14 (IP:15771-1-AP, 4ug; Detection:15771-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human skin using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15771-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

15771-1-AP bindet in WB, IP, IHC,ELISA VAC14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAC14 fusion protein Ag8415
• Vollständiger Name: Vac14 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa, 65 kDa
• GenBank-Zugangsnummer: BC007214
• Gene symbol: VAC14
• Gene ID (NCBI): 55697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Exp Cell Res; Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.; Authors - Shin Morioka ; KD Validated
	WB	Virology; Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry.; Authors - Shirley Qiu ; KO Validated