Ubiquilin 1 Polyklonaler Antikörper

Ubiquilin 1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22126-1-AP

Synonyme

DA41, DSK2, hPLIC 1, PLIC 1, PLIC1, ubiquilin 1, UBQLN1, XDRP1

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 22126-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 22126-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 22126-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 22126-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 22126-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, L02-Zellen, Rattenherzgewebe, Rattenlebergewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22126-1-AP bindet in WB, IF, ELISA Ubiquilin 1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ubiquilin 1 fusion protein Ag17530
• Vollständiger Name: ubiquilin 1
• Berechnetes Molekulargewicht: 589 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-71 kDa
• GenBank-Zugangsnummer: BC010066
• Gene symbol: UBQLN1
• Gene ID (NCBI): 29979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.