1A6/DRIM Polyklonaler Antikörper

1A6/DRIM Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18830-1-AP

Synonyme

DRIM, NNP73, Novel nucleolar protein 73, Protein Key 1A6, UTP20

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 18830-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18830-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18830-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18830-1-AP

  IP Result of anti-1A6/DRIM (IP:18830-1-AP, 4ug; Detection:18830-1-AP 1:800) with HeLa cells lysate 4000ug.

• 

  IF Staining of HeLa using 18830-1-AP

  Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 18830-1-AP

  Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 18830-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18830-1-AP (1A6/DRIM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Eierstockgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18830-1-AP bindet in WB, IP, IF,ELISA 1A6/DRIM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: UTP20, small subunit (SSU) processome component, homolog (yeast)
• Berechnetes Molekulargewicht: 318 kDa
• Beobachtetes Molekulargewicht: 310 kDa
• GenBank-Zugangsnummer: NM_014503
• Gene symbol: UTP20
• Gene ID (NCBI): 27340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UTP20, also named as DRIM, interacts with KIAA0649.