USP9X Polyklonaler Antikörper

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• KD/KO Validated

USP9X Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-55054

Synonyme

Deubiquitinating enzyme FAF X, DFFRX, FAF, FAM, Fat facets in mammals, hFAM, Ubiquitin thiolesterase FAF X, USP9, USP9X

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-55054

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 USP9X antibody (CL594-55054) at dilution of 1:200.

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  FC experiment of HeLa using CL594-55054

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human USP9X (CL594-55054) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-55054 bindet in IF, FC (Intra) USP9X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 9, X-linked
• Berechnetes Molekulargewicht: 292 kDa
• Beobachtetes Molekulargewicht: 260-290 kDa
• GenBank-Zugangsnummer: NM_001039590
• Gene symbol: USP9X
• Gene ID (NCBI): 8239
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C