USP8 Polyklonaler Antikörper

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• KD/KO Validated

USP8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 27791-1-AP

Synonyme

Deubiquitinating enzyme 8, hUBPy, HumORF8, KIAA0055, Ubiquitin isopeptidase Y, ubiquitin specific peptidase 8, Ubiquitin thiolesterase 8, UBPY, USP8

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Galerie der Validierungsdaten

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  WB analysis using 27791-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27791-1-AP

  WB result of USP8 antibody (27791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP8 transfected HeLa cells.

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  WB analysis of MCF-7 using 27791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27791-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 27791-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

27791-1-AP bindet in WB, IHC, IF, ELISA USP8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP8 fusion protein Ag27143
• Vollständiger Name: ubiquitin specific peptidase 8
• Berechnetes Molekulargewicht: 1118 aa, 128 kDa
• Beobachtetes Molekulargewicht: 128 kDa
• GenBank-Zugangsnummer: BC110590
• Gene symbol: USP8
• Gene ID (NCBI): 9101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Differ; Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer; Authors - Hanshen Yang
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
mouse,rat	WB	Aging Dis; Exogenous H2S Promoted USP8 Sulfhydration to Regulate Mitophagy in the Hearts of db/db Mice.; Authors - Yu Sun
human	WB,IHC	Cancer Manag Res; Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.; Authors - Xu Jing ; KD Validated
human	WB	Front Oncol; Knockdown of USP8 inhibits prostate cancer cell growth, proliferation, and metastasis and promotes docetaxel’s activity by suppressing the NF-kB signaling pathway; Authors - Md. Tariqul Islam ; KD Validated
mouse	WB	Cancers (Basel); The Expression of Cell Cycle-Related Genes in USP8-Mutated Corticotroph Neuroendocrine Pituitary Tumors and Their Possible Role in Cell Cycle-Targeting Treatment; Authors - Beata Joanna Mossakowska