USP50 Polyklonaler Antikörper

USP50 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20374-1-AP

Synonyme

Inactive ubiquitin carboxyl-terminal hydrolase 50, USP50

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20374-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20374-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20374-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 20374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20374-1-AP (USP50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberzirrhosegewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

20374-1-AP bindet in WB, IHC, IF, ELISA USP50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 50
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_203494
• Gene symbol: USP50
• Gene ID (NCBI): 373509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function through targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.

Publikationen

Species	Application	Title
human,mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li ; KD Validated
human,mouse	WB	EMBO Rep; Vitamin C promotes ACE2 degradation and protects against SARS-CoV-2 infection; Authors - Yibo Zuo ; KD Validated
human	WB	J Investig Med; Induction of deubiquitinating enzyme USP50 during erythropoiesis and its potential role in the regulation of Ku70 stability.; Authors - Junting Cai