USP36 Monoklonaler Antikörper

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• KD/KO Validated

USP36 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1B3

Rezensionen (2)

Kat-Nr. : 68165-1-Ig

Synonyme

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

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Galerie der Validierungsdaten

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  WB analysis using 68165-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68165-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68165-1-Ig

  WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68165-1-Ig bindet in WB, ELISA USP36 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP36 fusion protein Ag6426
• Vollständiger Name: ubiquitin specific peptidase 36
• Berechnetes Molekulargewicht: 1123 aa, 123 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC038983
• Gene symbol: USP36
• Gene ID (NCBI): 57602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chrysa (Verified Customer) (02-01-2023)

Tested in HeLa cells in WB with control si and siRNA against USP36

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

USP36 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:68165-1-Ig)

FH

Chrysa (Verified Customer) (01-17-2023)

Unfortunately, due to couriers mistake the sample stayed 2 days at RT. I tried the antibody 3 times : 1/5000, 1:500 and 1/100 in 5%TBS-milk O/N at 4C in total lysates from HeLa cells (30μg) and didn't work (image uploaded, all the other antibodies worked in the same membrane e.g. anti-tubulin, anti-exosc10, anti-senp3). In 1/100 dilution I got non specific bands. Is it possible that the sample got degraded or is there anything I could do to optimize it to work?

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000, 1:500
• Cell Tissue Type: HeLa