USP33 Polyklonaler Antikörper

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• KD/KO Validated

USP33 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 20445-1-AP

Synonyme

Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1

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Galerie der Validierungsdaten

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  WB analysis using 20445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20445-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20445-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20445-1-AP

  IP Result of anti-USP33 (IP:20445-1-AP, 4ug; Detection:20445-1-AP 1:300) with HEK-293 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20445-1-AP (USP33 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20445-1-AP (USP33 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human kidney using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of U2OS using 20445-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 20445-1-AP (USP33 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

20445-1-AP bindet in WB, IP, IHC, IF, ELISA USP33 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP33 fusion protein Ag14268
• Vollständiger Name: ubiquitin specific peptidase 33
• Berechnetes Molekulargewicht: 942 aa, 107 kDa
• Beobachtetes Molekulargewicht: 103 kDa, 107 kDa
• GenBank-Zugangsnummer: BC016663
• Gene symbol: USP33
• Gene ID (NCBI): 23032
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing. This antibody is specific to USP33.

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	IF, WB	J Cell Biol; Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.; Authors - Jacob A Culver
mouse	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu
human,mouse	WB,IP	EMBO Rep; High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.; Authors - Yukang Yuan ; KD Validated
human	WB	Cell Death Dis; NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.; Authors - Min Li ; KD Validated
human	WB	Oncotarget; NEURL4 regulates the transcriptional activity of tumor suppressor protein p53 by modulating its oligomerization.; Authors - Monica Cubillos-Rojas ; KD Validated