USP28 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP28 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 17707-1-AP

Synonyme

Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17707-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17707-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 17707-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17707-1-AP

  IP Result of anti-USP28 (IP:17707-1-AP, 4ug; Detection:17707-1-AP 1:600) with HeLa cells lysate 2800ug.

• 

  IHC staining of human breast cancer using 17707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Mausherzgewebe, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

17707-1-AP bindet in WB, IP, IHC, IF, ELISA USP28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP28 fusion protein Ag12003
• Vollständiger Name: ubiquitin specific peptidase 28
• Berechnetes Molekulargewicht: 583aa,66 kDa; 1077aa,120 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC065928
• Gene symbol: USP28
• Gene ID (NCBI): 57646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

Publikationen

Species	Application	Title
mouse	WB,IHC	J Clin Invest; The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.; Authors - Markus E Diefenbacher ; KD Validated
human	WB	Cell Death Differ; Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.; Authors - Yunhua Peng ; KO Validated
human	IF	EMBO J; ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.; Authors - Lauren T Evans
human	IHC	Cell Rep; The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells.; Authors - Wu Yadi Y
human	IF	J Cell Biol; A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis.; Authors - Bramwell G Lambrus
human,mouse	WB,IHC	J Biol Chem; The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells; Authors - Junjun Li ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (11-10-2019)

Detect endogenous USP28 in A549 cell line

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: A549

USP28 Antibody Western Blot, validation (1:1500 dilution) in A549 (Cat no:17707-1-AP)