USP25 Polyklonaler Antikörper

USP25 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC

Konjugation

Unkonjugiert

Kat-Nr. : 28817-1-AP

Synonyme

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

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Galerie der Validierungsdaten

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  WB analysis of HEK-293T using 28817-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 28817-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 28817-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, PC-3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28817-1-AP bindet in WB, IHC USP25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP25 fusion protein Ag30463
• Vollständiger Name: ubiquitin specific peptidase 25
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 122-138 kDa
• GenBank-Zugangsnummer: BC015930
• Gene symbol: USP25
• Gene ID (NCBI): 29761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as a deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.