USP22 Polyklonaler Antikörper

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• KD/KO Validated

USP22 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 55110-1-AP

Synonyme

Deubiquitinating enzyme 22, KIAA1063, Ubiquitin thiolesterase 22, USP22, USP3L

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Galerie der Validierungsdaten

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  WB analysis using 55110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55110-1-AP (USP22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55110-1-AP

  WB result of USP22 antibody (55110-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP22 transfected A549 cells.

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  IP experiment of HepG2 using 55110-1-AP

  IP result of anti-USP22 (IP:55110-1-AP, 4ug; Detection:55110-1-AP 1:500) with HepG2 cells lysate 4000 ug.

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  IHC staining of human lung cancer using 55110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55110-1-AP (USP22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55110-1-AP (USP22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55110-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA USP22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 22
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 58-60 kDa
• GenBank-Zugangsnummer: NM_015276
• Gene symbol: USP22
• Gene ID (NCBI): 23326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.

Publikationen

Species	Application	Title
human	WB	Sci Adv; PMID: 29732405; Authors - Qingxiang Liu
mouse	WB	Nat Commun; ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.; Authors - Francesca Bufalieri
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
rat	WB,IF	Eur J Pharmacol; USP22 aggravated diabetic renal tubulointerstitial fibrosis progression through deubiquitinating and stabilizing Snail1; Authors - Xilin Zhao ; KD Validated
human	WB,CoIP	Oncol Rep; Targeting USP22 with miR‑30‑5p to inhibit the hypoxia‑induced expression of PD‑L1 in lung adenocarcinoma cells.; Authors - Xiaoyang Hua
rat	WB	Endocrinology; AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells; Authors - Kai-Peng Huang ; KD Validated