USP15 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

USP15 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1C9G3

Rezensionen (1)

Kat-Nr. : 67557-1-Ig

Synonyme

Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15

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Galerie der Validierungsdaten

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  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of 4T1 using 67557-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67557-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

67557-1-Ig bindet in WB, IHC, IF, ELISA USP15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP15 fusion protein Ag5712
• Vollständiger Name: ubiquitin specific peptidase 15
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 112 kDa
• GenBank-Zugangsnummer: BC020688
• Gene symbol: USP15
• Gene ID (NCBI): 9958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IF	Cell Death Dis; The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression.; Authors - Xiaohong Xia ; KD Validated
human	WB	Endocr Relat Cancer; LncRNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer; Authors - Xiaoli Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shunbin (Verified Customer) (06-07-2023)

Great antibody for Western Blot. Very specific single band

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000

USP15 Antibody Western Blot validation (1:5,000 dilution) in (Cat no:67557-1-Ig)