USP13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP13 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 16840-1-AP

Synonyme

Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP13 transfected HeLa cells.

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  WB analysis using 16840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16840-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP, 1:1000) with si-Control and si-USP13 transfected HeLa cells.

• 

  WB analysis of HeLa using 16840-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 16840-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16840-1-AP

  IP Result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16840-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16840-1-AP (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, HEK-293-Zellen, Maushodengewebe, PC-3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautkrebsgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16840-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA USP13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP13 fusion protein Ag10527
• Vollständiger Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Berechnetes Molekulargewicht: 863 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC016146
• Gene symbol: USP13
• Gene ID (NCBI): 8975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Genes Dev; Chaperone-mediated autophagy degrades mutant p53.; Authors - Vakifahmetoglu-Norberg Helin H
human,mouse	WB,IHC,IF	EBioMedicine; The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.; Authors - Lian Li ; KD Validated
mouse,human	WB,IF	Transl Res; Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.; Authors - Xinxin Liao ; KO Validated
human	WB	J Cell Biol; Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.; Authors - Hong-Guang Xia
human	WB,IP	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma ; KD Validated
mouse	IP	J Cell Physiol; IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.; Authors - Lian Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Francesca (Verified Customer) (05-22-2019)

Very good antibody for western blot. Antibody specificity validated by USP13 knock-down.

• Applications: Western Blot,
• Primary Antibody Dilution: 1: 2000 in 5% milk
• Cell Tissue Type: MeT-5A cells

USP13 Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16840-1-AP)