USHBP1 Polyklonaler Antikörper

USHBP1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17226-1-AP

Synonyme

AIE 75 binding protein, AIEBP, MCC 2, MCC2, USH1C binding protein 1, USHBP1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 17226-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 17226-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of COLO 320 using 17226-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 17226-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 17226-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17226-1-AP (USHBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17226-1-AP bindet in WB, IHC, IF,ELISA USHBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USHBP1 fusion protein Ag11064
• Vollständiger Name: Usher syndrome 1C binding protein 1
• Berechnetes Molekulargewicht: 703 aa, 76 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC027910
• Gene symbol: USHBP1
• Gene ID (NCBI): 83878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USHBP1, also named as Mutated in colon cancer protein 2 or AIE-75-binding protein, is a 703 amino acid protein, which belongs to the MCC family. Highest level of expression is in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.