USF1 Monoklonaler Antikörper

USF1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8F1

Kat-Nr. : 67114-1-Ig

Synonyme

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

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Galerie der Validierungsdaten

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  WB analysis using 67114-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67114-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67114-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67114-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67114-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67114-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67114-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HT-29-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67114-1-Ig bindet in WB, ELISA USF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USF1 fusion protein Ag17785
• Vollständiger Name: upstream transcription factor 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: BC035505
• Gene symbol: USF1
• Gene ID (NCBI): 7391
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa