URG4 Monoklonaler Antikörper

URG4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5F12

Kat-Nr. : 67144-1-Ig

Synonyme

KIAA1507, up regulated gene 4, URG4, URGCP

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Galerie der Validierungsdaten

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  WB analysis using 67144-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67144-1-Ig (URG4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human nasopharyngeal carcinoma using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67144-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using URG4 antibody (67144-1-Ig, Clone: 2D5F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, HepG2-Zellen, HuH-7-Zellen, MKN-45-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Nasopharynxkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67144-1-Ig bindet in WB, IHC, IF, ELISA URG4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: URG4 fusion protein Ag28635
• Vollständiger Name: up-regulated gene 4
• Berechnetes Molekulargewicht: 922 aa, 104 kDa
• Beobachtetes Molekulargewicht: 104 kDa
• GenBank-Zugangsnummer: BC018426
• Gene symbol: URG4
• Gene ID (NCBI): 55665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.