UQCRFS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UQCRFS1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 18443-1-AP

Synonyme

Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1

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Galerie der Validierungsdaten

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  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18443-1-AP

  IP Result of anti-UQCRFS1 (IP:18443-1-AP, 4ug; Detection:18443-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 2 publications below

Produktinformation

18443-1-AP bindet in WB, IP, IHC, ELISA UQCRFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UQCRFS1 fusion protein Ag13315
• Vollständiger Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC010035
• Gene symbol: UQCRFS1
• Gene ID (NCBI): 7386
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
pig	WB	J Cachexia Sarcopenia Muscle; MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.; Authors - Ming Qi
human,mouse	WB	Cell Biosci; Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth; Authors - Hongting Zhao
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu
human	WB	Int J Mol Sci; Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells; Authors - Harpreet Kaur

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

ZEE (Verified Customer) (07-13-2020)

WE TRY THIS ANTIBODY WITH MOUSE HEART SAMPLES, AND GOT NICE RESULTS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: ADULT MOUSE HEART