UQCRC2 Monoklonaler Antikörper

UQCRC2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1A5G11

Kat-Nr. : 67547-1-Ig

Synonyme

Complex III subunit 2, Core protein II, QCR2, UQCR2, UQCRC2

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Galerie der Validierungsdaten

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  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Hausschwein-Herzgewebe, Hausschwein-Hirngewebe, humanes Plazenta-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

67547-1-Ig bindet in WB, IHC, ELISA UQCRC2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UQCRC2 fusion protein Ag6874
• Vollständiger Name: ubiquinol-cytochrome c reductase core protein II
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000484
• Gene symbol: UQCRC2
• Gene ID (NCBI): 7385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li