UPK2 Polyklonaler Antikörper

UPK2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 21149-1-AP

Synonyme

UP2, UPII, upk 2, UPK2, uroplakin 2, Uroplakin II

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 21149-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21149-1-AP (UPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21149-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21149-1-AP (UPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

21149-1-AP bindet in WB, IHC, IF, ELISA UPK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPK2 fusion protein Ag15694
• Vollständiger Name: uroplakin 2
• Berechnetes Molekulargewicht: 184 aa, 19 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC113900
• Gene symbol: UPK2
• Gene ID (NCBI): 7379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-2 (UPK2) is a single-pass type I membrane protein. UPK2 may play an important role in regulating the assembly of the AUM.

Publikationen

Species	Application	Title
human	IF	Biofabrication; Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment; Authors - Changhao Hou
human	IF	Sci Rep; Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder.; Authors - Yuta Inoue
rat	WB,IHC	Int J Mol Sci; Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2-Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat; Authors - Hidenori Akaihata