UPK1A Polyklonaler Antikörper

UPK1A Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25275-1-AP

Synonyme

Tetraspanin 21, Tspan 21, TSPAN21, UP1A, UPIA, UPK1A, UPKA, uroplakin 1A, Uroplakin Ia

---

Galerie der Validierungsdaten

• 

  IP experiment of mouse bladder using 25275-1-AP

  IP Result of anti-UPK1A (IP:25275-1-AP, 4ug; Detection:25275-1-AP 1:300) with mouse bladder tissue lysate 3200ug.

• 

  IHC staining of human bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 10x lens)..

• 

  IHC staining of human bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 40x lens)..

• 

  IHC staining of mouse bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche IP: Maus-Blasengewebe
• Erfolgreiche Detektion in IHC: humanes Blasengewebe, Maus-Blasengewebe, Ratten-Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25275-1-AP bindet in IP, IHC, IF, ELISA UPK1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPK1A fusion protein Ag17356
• Vollständiger Name: uroplakin 1A
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC107098
• Gene symbol: UPK1A
• Gene ID (NCBI): 11045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.

Publikationen

Species	Application	Title
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
human	IHC	Int J Urol; Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types.; Authors - Maki Kawasaki-Nanri