UPF3B Polyklonaler Antikörper

UPF3B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23301-1-AP

Synonyme

HUPF3B, hUpf3p X, MRXS14, RENT3B, UPF3B, UPF3X

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Galerie der Validierungsdaten

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  WB analysis using 23301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23301-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23301-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50.

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  IHC staining of human testis using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

23301-1-AP bindet in WB, IHC,ELISA UPF3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPF3B fusion protein Ag18568
• Vollständiger Name: UPF3 regulator of nonsense transcripts homolog B (yeast)
• Berechnetes Molekulargewicht: 483 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC121017
• Gene symbol: UPF3B
• Gene ID (NCBI): 65109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Front Genet; Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.; Authors - Zhen Zhang