UNC5C-Specific Polyklonaler Antikörper

UNC5C-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 20240-1-AP

Synonyme

Netrin receptor UNC5C, Protein unc 5 homolog 3, Protein unc 5 homolog C, unc 5 homolog C (C. elegans), UNC5C, UNC5C-Specific, UNC5H3

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Galerie der Validierungsdaten

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  WB analysis using 20240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20240-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20240-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20240-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20240-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20240-1-AP bindet in WB, IHC, ELISA UNC5C-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-5 homolog C (C. elegans)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 103 kDa
• GenBank-Zugangsnummer: NM_003728
• Gene symbol: UNC5C
• Gene ID (NCBI): 8633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.

Publikationen

Species	Application	Title
rat	WB	Alcohol Clin Exp Res; Fetal alcohol exposure impairs learning and memory functions and elevates levels of various biochemical markers of Alzheimer's disease in the brain of twelve-month-old rats; Authors - Shaista Chaudhary

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Devi (Verified Customer) (02-01-2019)

20ug mouse brain cortex homogenates (lane 1 and 3: WT and Lane 2: Unc5c KO) were loaded and probed with rabbit anti-Unc5c antibody at a dilution of 1:3000 with 5% milk in TBST for 1.5 hr at room temperature. Secondary antibody was used in 1:5000 dilution and developed using ECL west femto. The only concern was that KO also showed a band albeit a faint one. WT reprised the bands showedas expected although I have concerns about bands showing up in KO tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse brain cortex homogenate

UNC5C-Specific Antibody Western Blot, validation (1:3000 dilution) in Mouse brain cortex homogenate (Cat no:20240-1-AP)