UNC45A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UNC45A Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 19564-1-AP

Synonyme

GC UNC45, GCUNC 45, GCUNC45, IRO039700, Protein unc 45 homolog A, SMAP 1, SMAP1, unc 45 homolog A (C. elegans), Unc 45A, UNC45A

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 19564-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19564-1-AP

  WB result of UNC45A antibody (19564-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

• 

  WB analysis of mouse lung using 19564-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19564-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 19564-1-AP

  IP Result of anti-UNC45A (IP:19564-1-AP, 4ug; Detection:19564-1-AP 1:300) with K-562 cells lysate 3600ug.

• 

  IHC staining of human ovary tumor using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19564-1-AP (UNC45A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 19564-1-AP (UNC45A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 19564-1-AP (UNC45A antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, Mauslungengewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

19564-1-AP bindet in WB, IP, IHC, IF, ELISA UNC45A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-45 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_018671
• Gene symbol: UNC45A
• Gene ID (NCBI): 55898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.

Publikationen

Species	Application	Title
human	WB	FASEB J; A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.; Authors - Susana Lechuga ; KO Validated
rat	WB, IF	J Cell Sci; UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.; Authors - Juri Habicht
mouse	IHC,IF	Cells; UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.; Authors - Valentino Clemente

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kishor (Verified Customer) (10-17-2019)

This Ab works well for western blotting (1:1000) but I did not get satisfactory results for Immunofluorescence (1:200) in human liver cell lines.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: H69, HUCCT-1

UNC45A Antibody Western Blot,Immunofluorescence, validation (1:1000 and 1:200 dilution) in H69, HUCCT-1 (Cat no:19564-1-AP)