UNC13A/Munc13-1 Polyklonaler Antikörper

UNC13A/Munc13-1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55053-1-AP

Synonyme

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A, UNC13A/Munc13-1

---

Galerie der Validierungsdaten

• 

  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55053-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55053-1-AP

  IP Result of anti-UNC13A/Munc13-1 (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:500) with mouse brain tissue lysate 5200ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55053-1-AP bindet in WB, IP, CoIP, ELISA UNC13A/Munc13-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-13 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 193 kDa
• Beobachtetes Molekulargewicht: 193-210 kDa
• GenBank-Zugangsnummer: NM_001080421
• Gene symbol: UNC13A
• Gene ID (NCBI): 23025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Publikationen

Species	Application	Title
human	WB	Nature; TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.; Authors - X Rosa Ma
mouse	WB	Elife; Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.; Authors - Fushun Fan
	CoIP	Cell Rep; Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.; Authors - Hao Zhou