ULK1 Polyklonaler Antikörper

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• KD/KO Validated

ULK1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

53

Kat-Nr. : 20986-1-AP

Synonyme

ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 20986-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 20986-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Lebergewebe, humanes Leberkarzinomgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

20986-1-AP bindet in WB, IHC, IF, ELISA ULK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 113-140 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Publikationen

Species	Application	Title
human	IHC	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan ; KO Validated
human	WB	Autophagy; Topology-dependent, bifurcated mitochondrial quality control under starvation.; Authors - Yanshuang Zhou
human	IF	Dev Cell; Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner.; Authors - Cansu Karabiyik
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
mouse	WB	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane